Determines the level of a target even though it may be in very low concentrations. Advantages include the ability to adapt this assay to test for latex glove antigen (allergen) by utilizing a known amount of IgE antibodies to latex proteins derived from pooled serum; results expressed as mg/gm or ppm (allergen/gram of glove). The major disadvantage is that the level of antibody present is not necessarily directly related to the severity of the allergic response; an individual can have very little IgE and still have a severe response. SEE: ELISA